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Abstract #0460

Detection of macrophage-based inflammation following renal ischemia reperfusion injuries using super-paramagnetic iron oxide (SPIO) nanoparticles in T2-weighted MRI.

B G Hammond 1 , J C Montejano 2 , J M Poth 2 , K M Huber 2 , M Stukova 2 , D Golovko 3 , and N J Serkova 2

1 University of Arizona College of Medicine - Phoenix, Phoenix, Arizona, United States, 2 University of Colorado Anschutz Medical Campus, Aurora, Colorado, United States, 3 Good Samaritan Medical Center, Massachusetts, United States

Following renal ischemia, reperfusion injuries occur, mediated by a macrophage inflammatory response. Due to the role of macrophages in iron metabolism it is possible to image inflammation using iron oxide nano particles (SPIONs) and T2w-MRI. In this study, acute kidney injury was induced in 10 female C57 mice by clamping the renal artery. Clodronate (CLD) driven macrophage depletion was attempted. In developing this protocol, we have shown that CLD is not an appropriate agent to deplete macrophages in the kidney and that 24hours is not an appropriate time point to assess macrophages using SPION T2-MRI due to their disturbed kinetics.

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