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Abstract #0526

Reduced production of hyperpolarized 5-13C-glutamate is associated with the IDH1 mutation

Jose Luis Izquierdo Garcia 1 , Pavithra Luis Viswanath 1 , Pia Eriksson 1 , Marina Radoul 1 , Larry Cai 1 , Myriam M Chaumeil 1 , Russell O Pieper 2 , Joanna J Phillips 2 , and Sabrina M Ronen 1

1 University California San Francisco, San Francisco, California, United States, 2 Department of Neurological Surgery, Helen Diller Research Center, University California San Francisco, San Francisco, California, United States

This study investigated the role that mutant IDH1-driven metabolic reprogramming plays in tumorigenesis. We investigated hyperpolarized 5-13C-glutamate production from hyperpolarized 2-13C-pyruvate in mutant and wild-type cells and found that glutamate production was significantly reduced in mutant cells, associated with a drop in PDH activity. Treatment with dichloroacetate, a PDH agonist, significantly increased HP 5-13C-glutamate production with an accompanying decrease in clonogenicity. Our results indicate that HP 5-13C-glutamate can serve as a metabolic imaging biomarker of PDH down-regulation in IDH1 mutant glioma cells.

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