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Abstract #0613

Resolving Cellular Specific Microarchitectures Using Double Pulsed Field Gradient Weighted, Relaxation-Enhanced Magnetic Resonance Spectroscopy

Noam Shemesh 1 , Jens T Rosenberg 2,3 , Jean-Nicolas Dumez 4 , Lucio Frydman 2,5 , and Samuel C Grant 2,3

1 Champalimaud Neuroscience Programme, Champalimaud Centre for the Unknown, Lisbon, Portugal, 2 National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, United States, 3 Chemical & Biomedical Engineering, Florida State University, Tallahassee, FL, United States, 4 Institut de Chimie des Substances Naturelles, CNRS, UPR2301, Gif-sur-Yvette, France, 5 Chemical Physics, Weizmann Institute of Science, Rehovot, Israel

Cellular-specific microarchitectures are altered with neurodegeneration and neuroplasticity, yet their characterization remains elusive, especially because most diffusion MR techniques rely on ubiquitous water signals. Here, we present a methodology capable of depicting cellular-specific microarchitectures in vivo. We employ Relaxation Enhanced 1H MRS at 21.1 T, and selectively target N-Acetylaspartate and myo-Inositol resonances as markers for neurons and astrocytes, respectively. When coupled with a double Pulsed Field Gradient filter, RE-MRS provides the sensitivity required for characterization of cellular-specific morphologies. Randomly oriented cell processes were selectively detected. These findings provide a framework for future characterizations of diseased and healthy tissues.

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