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Abstract #0682

Validation of MRI microstructure measurements with Coherent Anti-Stokes Raman Scattering (CARS)

Tanguy Duval 1 , Alicja Gasecka 2,3 , Philippe Pouliot 1,4 , Daniel Ct 2,3 , Nikola Stikov 1,5 , and Julien Cohen-Adad 1,6

1 Institute of Biomedical Engineering, Polytechnique Montral, Montral, Qc, Canada, 2 Quebec Mental Health University Institute, Qubec, Qc, Canada, 3 Doptic, photonic and laser Center, Universit Laval, Qubec, Qc, Canada, 4 Montreal Heart Institute, Montreal, Quebec, Canada, 5 Montreal Neuronal Institute, McGill University, Montral, Qubec, Canada, 6 Functional Neuroimaging Unit, CRIUGM, Universit de Montral, Montral, Qc, Canada

Histology is considered to be the gold standard for validating quantitative MRI techniques. However, common histology techniques can induce biases due to the tissue preparation (fixation, cutting and staining), rendering challenging the validation of quantitative MRI biomarkers. Recently, a large-scale microscopy technique called Coherent Anti-Stokes Raman Scattering (CARS) was introduced, enabling (i) sub-micrometer resolution, (ii) in-depth focus preventing artifacts related to non-perfectly flat tissue surface and (iii) specific contrast to myelin without the need for staining. Here we applied the CARS technique to validate AxCaliber experiments for estimating axon diameter measurements.

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