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Abstract #1748

Bound water in reconstructed skin samples: quantification by NMR

Genevive Guillot 1 , Sarah Risquez 1 , Chih-Ying Wang 1 , Jean-Baptiste Galey 2 , Marion Ghibaudo 2 , and Bernard Querleux 2

1 CNRS Univ Paris-Sud, IR4M UMR8081, ORSAY, France, 2 L'Oreal Research & Innovation, AULNAY-SOUS-BOIS, France

Reconstructed skin samples are routinely used for in vitro safety and efficacy evaluation of cosmetic ingredients. MT-NMR is well-suited as a non-destructive technique for the quantification of the bound proton fraction. In this work, an off-resonance MT-NMR sequence was used to characterize dermis from reconstructed skin samples. A linear relationship between the bound proton fraction and the solid weight fraction was found with a slope compatible with 0.2 g of bound water per g of collagen, in fair agreement with the 4 water molecules per tripeptide description of collagen hydration.

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