Abstract #1890
Low cell number perfusion bioreactor system for hyperpolarized MRS in a MRI setting
Lotte Bonde Bertelsen 1 , Simon Lauritsen 1 , Christoffer Laustsen 1 , Preben Daugaard 1 , Xiaolu Zhang 1 , and Hans Stdkilde-Jrgensen 1
1
The MR Research Centre, Department of
Clinical Medicine, Aarhus University, Aarhus University
Hospital, Aarhus, Denmark
Development of a MR-compatible bioreactor system for
measuring metabolic fluxes in cells grown in scaffold
makes it possible to examine various cell types with
different hyperpolarized
13
C
bio-probes. The 3D printed scaffold system facilitates a
dynamic cell culture environment with controlled
nutrient flow, oxygen supply and a large surface area
for growth of adherent cells with minimal disturbance of
the cells. Ongoing studies are focusing on evaluation of
low cell numbers in scaffold and in suspension in this
bioreactor design.
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