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Abstract #1928

In vivo quantification of human natural killer cells by 19 F MRI

Kai D. Ludwig 1 , Myriam Bouchlaka 2 , Jeremy Gordon 1 , Christian Capitini 2 , and Sean B. Fain 1,3

1 Medical Physics, University of Wisconsin-Madison, Madison, WI, United States, 2 Pediatrics and Carbone Cancer Center, University of Wisconsin-Madison, Madison, WI, United States, 3 Radiology and Biomedical Engineering, University of Wisconsin-Madison, WI, United States

A current unmet need in cellular-based immunotherapies is longitudinal tracking and quantification of cells after infusion into a patient. Previously, human natural killer (hNK) cells have successfully been intracellularly labeled with a perfluoropolyether (PFPE) enabling in vivo detection and longitudinal tracking by 19 F MRI. Here, a labeling concentration of 4mg/mL PFPE resulted in hNKs with 3.6 plus-or-minus sign 0.5 multiplication sign 10 11 19 F/cell. A minimum detection limit was extrapolated to be 1.2 multiplication sign 10 5 hNKs/voxel at 4.7 T. In vivo quantification on a tumor-bearing mouse showed intratumorally localized 19 F signal over 7 days with 60-70% of the initially injected hNK cells being detected at all time points.

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