Proton NMR-based Metabolomic Profiling in Pulmonary Tuberculosis Patients
Savita Singh 1 , Sujeet Mewar 2 , Deepti Upadhyay 2 , Uma Sharma 2 , Anand Jaiswal 3 , Rohit Sarin 3 , Naranamangalam R Jagannathan 2 , and H K Prasad 1
Department of Biotechnology, All India
Institute of Medical Sciences, New Delhi, Delhi, India,
of NMR and MRI Facility, All India Institute of Medical
Sciences, New Delhi, Delhi, India,
of TB and Respiratory Diseases, National Institute of
Tuberculosis and Respiratory Diseases, New Delhi, Delhi,
In this study, 1H NMR spectroscopy was employed to
identify metabolites present in sera of untreated
pulmonary tuberculosis patients (PTB =15); household
healthy contacts (HHC=13); healthy controls (HC=14); and
treated pulmonary tuberculosis patients (TPTB=13).
Oneway analysis of variance followed by Bonferroni test
was used for determining the statistical significance of
estimated metabolite concentrations. Of the 13
metabolites that showed significant difference between
various groups, citrate was found to be reduced in PTB
patients compared to all other groups. Our study
revealed that NMR based metabonomics may provide an
insight to understand the biochemical alteration(s) that
occur in PTB patients.
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