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Abstract #1990

Proton NMR-based Metabolomic Profiling in Pulmonary Tuberculosis Patients

Savita Singh 1 , Sujeet Mewar 2 , Deepti Upadhyay 2 , Uma Sharma 2 , Anand Jaiswal 3 , Rohit Sarin 3 , Naranamangalam R Jagannathan 2 , and H K Prasad 1

1 Department of Biotechnology, All India Institute of Medical Sciences, New Delhi, Delhi, India, 2 Department of NMR and MRI Facility, All India Institute of Medical Sciences, New Delhi, Delhi, India, 3 Department of TB and Respiratory Diseases, National Institute of Tuberculosis and Respiratory Diseases, New Delhi, Delhi, India

In this study, 1H NMR spectroscopy was employed to identify metabolites present in sera of untreated pulmonary tuberculosis patients (PTB =15); household healthy contacts (HHC=13); healthy controls (HC=14); and treated pulmonary tuberculosis patients (TPTB=13). Oneway analysis of variance followed by Bonferroni test was used for determining the statistical significance of estimated metabolite concentrations. Of the 13 metabolites that showed significant difference between various groups, citrate was found to be reduced in PTB patients compared to all other groups. Our study revealed that NMR based metabonomics may provide an insight to understand the biochemical alteration(s) that occur in PTB patients.

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