Abstract #1995
A high-resolution 2D J-resolved NMR method for intact biological samples
Yuqing Huang 1 , Chunhua Tan 1 , Shuhui Cai 1 , and Zhong Chen 1
1
Electronic Science, Xiamen University,
Xiamen, Fujian, China
2D J-resolved spectroscopy (JRES) is an alternative to
1D NMR for the metabolite analysis due to its ability on
separating congested signals along two frequency
dimensions. However, conventional 2D JRES approaches are
generally subjected to field inhomogeneous effects from
macroscopic magnetic susceptibility in biological
samples. In this report, an NMR acquisition method based
on intermolecular single-quantum coherences (iSQCs) is
presented to achieve high-resolution 2D J-resolved
information directly from intact biological samples.
This method is merely based on pulse sequence design and
can work on standard NMR spectrometers without any
hardware requirement.
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