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Abstract #1995

A high-resolution 2D J-resolved NMR method for intact biological samples

Yuqing Huang 1 , Chunhua Tan 1 , Shuhui Cai 1 , and Zhong Chen 1

1 Electronic Science, Xiamen University, Xiamen, Fujian, China

2D J-resolved spectroscopy (JRES) is an alternative to 1D NMR for the metabolite analysis due to its ability on separating congested signals along two frequency dimensions. However, conventional 2D JRES approaches are generally subjected to field inhomogeneous effects from macroscopic magnetic susceptibility in biological samples. In this report, an NMR acquisition method based on intermolecular single-quantum coherences (iSQCs) is presented to achieve high-resolution 2D J-resolved information directly from intact biological samples. This method is merely based on pulse sequence design and can work on standard NMR spectrometers without any hardware requirement.

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