Meeting Banner
Abstract #2032

Etomidate: A novel anesthetic of choice for functional magnetic resonance imaging in mice

Georges Hankov* 1,2 , Marija M Petrinovic* 1 , Aileen Schroeter 2 , Andreas Bruns 1 , Markus Rudin 2,3 , Markus von Kienlin 1 , Basil Knnecke 1 , and Thomas Mueggler 1

1 Neuroscience Discovery, F. Hoffmann-La Roche Pharmaceuticals Ltd, Basel, Basel-City, Switzerland, 2 Institute for Biomedical Engineering, University of Zurich and ETH, Zurich, Zurich, Switzerland, 3 Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland

Functional MRI applied across different genetic mouse models of neurodevelopmental disorders holds promise to reveal pathological alteration at the neurocircuitry level and thus to aid devising novel therapies and biomarkers. Prerequisite is a reliable, non-terminal anesthesia protocol that is applicable to longitudinal studies in a broad range of mouse lines. Here, a novel anesthesia protocol based on the injectable GABAergic anesthetic etomidate has been established and validated using perfusion and BOLD fMRI. Under etomidate, basal perfusion remains in a physiological range, cerebrovascular reserve capacity is preserved and regions-specific perfusion- and BOLD changes are elicited upon pharmacological and sensory stimulation, respectively.

This abstract and the presentation materials are available to members only; a login is required.

Join Here