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Abstract #2035

A reproducible experimental protocol for longitudinal rat fMRI studies: electrical mystacial pad stimulation under isoflurane anesthesia

Shin-Lei Peng 1,2 , Ling-Yi Huang 1 , Sheng-Min Huang 1 , Yi-Chun Wu 3 , Hanzhang Lu 2 , Fu-Chan Wei 4 , Chih-Jen Wen 4 , Hui-Yu Cheng 4 , Chih-Hung Lin 4 , and Fu-Nien Wang 1

1 Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, Taiwan, 2 Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, Texas, United States, 3 Molecular Imaging Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan, 4 Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Taoyuan, Taiwan

fMRI plays an role in neuroscience researches. Investigating animal fMRI studies is an essential step toward understandings of cortical reorganization after surgery. Most researches use alpha-chloralose as an anesthetic, which is improper for follow-up studies. Here, we established a new protocol of rat electric mystacial pad (MP) stimulation under isoflurane anesthesia with advantages of increased BOLD sensitivity and possibility for longitudinal studies. Results show MP stimulation exhibited reproducible activations in the sometosensory cortex. In terms of the extension of activations and induced BOLD signal changes, results from MP stimulation under isoflurane anesthesia are comparable with those under alpha-chloralose anesthesia.

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