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Abstract #2042

Combined optogenetic fMRI and optical Ca2+-recordings for functional mapping of thalamo-cortical circuits in rat

Lydia Wachsmuth 1 , Florian Schmid 1 , Miriam Schwalm 2 , Albrecht Stroh 2 , and Cornelius Faber 1

1 Department of Clinical Radiology, University of Mnster, Mnster, Germany, 2 Institute of Microscopic Anatomy and Neurobiology, Johannes Gutenberg-University Mainz, Mainz, Germany

We combined small animal fMRI with fiber-based optogenetic excitation of CHR2 and C1V1, transduced in rat cortex and thalamus, and with cortical fluorescence detection of a Ca2+-sensitive dye (OGB-1) in order to assess the scale of optogenetic network activation in terms of the spatial and temporal patterns of network recruitment. Onset, peak time and decay characteristics of BOLD time courses and amplitude and decay characteristic of Ca2+ responses appeared similar upon optogenetic and sensory-driven, endogenous network activation, confirming that ofMRI can serve as a model for functional mapping of brain circuits.

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