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Abstract #2603

In vivo Assessment of Free Radicals in a Mouse Model for Diabetic Cardiomyopathy

Rheal A. Towner 1 , Nataliya Smith 1 , Jorge Carrizales 1 , Debra Sauners 1 , Robert Silasi-Mansat 2 , Florea Lupu 2 , Marilyn Ehrenshaft 3 , and Ronald P. Mason 3

1 Advanced Magnetic Resonance Center, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States, 2 Cardiovascular Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States, 3 NIEHS, NC, United States

Cardiovascular disease is the primary cause of morbidity and mortality among the diabetic population. One of the characteristics associated with diabetic cardiomyopathy is the generation of free radicals. In this study we incorporated a spin trapping compound, DMPO, to trap free radicals in STZ-induced diabetic mice [2], and used a trapped free radical-targeted molecular MRI probe (anti-DMPO probe) to detect in vivo levels of free radicals in cardiac muscle of diabetic mice. There was a significant increase in the percent change in MRI signal intensity in diabetic mouse hearts (p<0.01) compared to non-diabetic mice, both administered DMPO and the anti-DMPO free radical-targeted probe. The biotin moiety on the anti-DMPO probe was used to confirm its presence in excised tissue with streptavidin-Cy3. In conclusion, a free radical-targeted molecular MRI can be used to detect in vivo heterogeneous levels of free radicals in a mouse model for diabetic cardiomyopathy.

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