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Abstract #3041

Micro-MR correlates of cellular-level alterations in epileptogenesis

Katharina Gbel 1 , Johannes Gerlach 2 , Robert Kamberger 3 , Jochen Leupold 1 , Dominik von Elverfeldt 1 , Carola Haas 2 , Jan G. Korvink 3 , Jrgen Hennig 1 , and Pierre LeVan 1

1 Medical Physics, Dept. of Radiology, University Medical Center Freiburg, Freiburg, Germany, 2 Experimental Epilepsy Research, University Medical Center Freiburg, Freiburg, Germany, 3 Dept. of Microsystems Engineering (IMTEK), Technical Faculty, University of Freiburg, Freiburg, Germany

Organotypic hippocampal slice cultures are a well established neuronal culture system that combines the advantages of cell culturing with a neuronal network tightly reflecting the in vivo state. Our aim is to investigate these changes during epileptogenesis using high spatial resolution MR microscopy, whose non invasiveness allows continuous longitudinal monitoring. GRE and EPI-DTI sequence protocols were adapted in order to resolve hippocampal cytoarchitecture and connectivity patterns of epileptic and healthy tissue and a correspondence was established with immunohistochemical changes. This provides deeper insights into the dynamic processes of epileptogenesis at the cellular level in in vitro preparations.

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