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Abstract #0382

Multiband Spectral-Spatial RF Excitation for Hyperpolarized [2‑13C]Dihydroxyacetone 13C-MR Metabolism Studies

Irene Marco-Rius1, Peng Cao1, Cornelius von Morze1, Matthew Merritt2, Karlos X Moreno3, Gene-Yuan Chang4, Michael A Ohliger1, David Pearce4, John Kurhanewicz1, Peder EZ Larson1, and Daniel B Vigneron1

1Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA, United States, 2Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL, United States, 3Department of Chemistry, Engineering, Pre-Pharmacy, and Physics, South Texas College, Weslaco, TX, United States, 4Department of Medicine, Division of Nephrology, University of California San Francisco, San Francisco, CA, United States

13C-MR spectra of hyperpolarized [2-13C]dihydroxyacetone (DHAc), a new agent for imaging gluconeogenesis, was acquired using specialized acquisition methods in the rat liver and kidney in vivo. Because the resonances originating from the metabolism of [2-13C]DHAc have a large frequency distribution, we designed a novel spectral-spatial (SPSP), multi-band excitation pulse that corrects for chemical shift misregistration, resulting in accurate spatial-spectral selectivity. The metabolic products phosphoenolpyruvate (PEP) and glycerol 3-phosphate (G3P) were detected, evidencing metabolism of the hyperpolarized substrate towards the glycolytic pathway and activity of the enzyme glycerol 3-phosphate dehydrogenase.

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