Abstract #1705

Light isoflurane sedation: an excellent trade-off between anesthesia and awake condition in functional connectivity studies with rats

Jaakko Paasonen¹, Raimo A Salo¹, Artem Shatillo², and Olli Gröhn¹

¹Department of Neurobiology, University of Eastern Finland, Kuopio, Finland, ²Charles River Discovery Services, Kuopio, Finland

Prevention of motion is a prerequisite for preclinical functional connectivity (FC) studies. However, anesthesia alters brain function, and awake protocols may induce stress. Therefore, we investigated the feasibility of using light sedation in FC studies. FC was estimated under 0.1/0.5% isoflurane (subanesthetic doses) with acclimatized rats, and under 1.3% isoflurane (anesthetic dose). Results demonstrate different FC between anesthetic and subanesthetic doses. The physiologic measures suggest, that the 0.5% rats adapted well to imaging, while the 0.1% rats did so insufficiently. Therefore, light isoflurane sedation may provide an excellent combination for FC investigations: minimal stress and motion with normal brain function.

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