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Abstract #2337

Concentration-dependent hepatic metabolism in vivo using a near physiological dose range of hyperpolarized [1-13C]pyruvate

Emine Can1, Jessica A. M. Bastiaansen2,3, Hikari A. I. Yoshihara4,5, Rolf Gruetter3,5,6, and Arnaud Comment1

1Institute of Physics of Biological Systems, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland, 2Department of Radiology, University Hospital Lausanne (CHUV), Lausanne, Switzerland, 3Department of Radiology, University of Lausanne (UNIL), Lausanne, Switzerland, 4Institute of Physics of Biological Systems, EPFL, Lausanne, Switzerland, 5Laboratory for Functional and Metabolic Imaging, EPFL, Lausanne, Switzerland, 6Department of Radiology, University of Geneva, Geneva, Switzerland

Hyperpolarized 13C-labeled pyruvate provides assessment of real-time liver mitochondrial enzymatic activities directly by labeling TCA cycle intermediates. However the technique is limited by the requirement of supraphysiological concentrations due to the low basal concentrations of metabolic intermediates. In this study we showed the feasibility of detecting liver metabolism in vivo with HP 13C pyruvate administered at plasma concentrations of at most 7-fold of the basal levels. Different metabolic response to the concentration change shows that the adaptation to supraphysiological levels can obscure feeding state-depending metabolic differences in liver.

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