Abstract #2340

13C dynamic nuclear polarization NMR for quantification of metabolic flux of endothelial progenitor cells

Nathalie Nielsen¹, Christoffer Laustsen¹, Hans Stødkilde-Jørgensen¹, and Lotte Bonde Bertelsen¹

¹MR Research Centre, Department of Clinical Medicine, Aarhus University Hospital, Aarhus University, Aarhus, Denmark

This study aims to quantify the metabolic flux in EPCs in order to characterize the metabolic changes occurring during in-vitro culturing utilized for cell expansion, 3D scaffolds and suspension. [1-¹³C] hyperpolarized pyruvate is injected to a NMR compatible bioreactor system and the conversion is detected and measured as the lactate/pyruvate ratio. Activation assays and qPCR is performed to support the results. The lactate/pyruvate (6±1,07 fold) and LDH activity is increased in cell suspension culturing. Together with an elevated PDH expression in suspension cultures our conclusion is that adherent cells metabolically compensate in the suspension culture due to the environmental conditions.

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