Nathalie Nielsen1, Christoffer Laustsen1, Hans Stødkilde-Jørgensen1, and Lotte Bonde Bertelsen1
This study aims to quantify the metabolic
flux in EPCs in order to characterize the metabolic changes occurring during in-vitro culturing utilized for cell
expansion, 3D scaffolds and suspension.
[1-13C] hyperpolarized pyruvate
is injected to a NMR compatible bioreactor system and the conversion is
detected and measured as the lactate/pyruvate ratio. Activation assays and qPCR
is performed to support the results.
The lactate/pyruvate (6±1,07
fold) and LDH activity
is increased in cell suspension culturing. Together with an elevated PDH
expression in suspension cultures our conclusion is that adherent cells
metabolically compensate in the suspension culture due to the environmental conditions.