Abstract #3680

Hyperpolarized 13C-labeled 5-5-dimethyl-1-pyrroline-N-oxide for in vivo detection of reactive oxygen species

Keita Saito¹, Shingo Matsumoto², Deepak Sail³, Shun Kishimoto¹, Hellmut Merkle⁴, Marcelino Bernardo⁵, Rolf Swenson³, James B. Mitchell¹, and Murali C. Krishna¹

¹Radiation Biology Branch, National Cancer Institute, Bethesda, MD, United States, ²Graduate School of Information Science and Technology, Hokkaido University, Sapporo, Japan, ³Imaging Probe Development Center, National Heart, Lung, and Blood Institute, Rockville, MD, United States, ⁴National Institute of Neurological Disorder and Stroke, Bethesda, MD, United States, ⁵Molecular Imaging Program, National Cancer Institute, Bethesda, MD, United States

5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) is a spin trap agent used to detect reactive oxygen species (ROS). We synthesized ¹³C-labeled DMPO, and investigated feasibility of hyperpolarized ¹³C-DMPO to detect ROS generated in living animals. Hyperpolarized ¹³C-DMPO gave us a single peak at 76 ppm on the ¹³C-spectrum, and ¹³C-DMPO was distributed through the mouse body immediately after intravenous injection. The results indicate hyperpolarized ¹³C-DMPO provided sufficient magnitude of the ¹³C signal to be detected in the mouse body, and can be applied to some disease models to evaluate the capability for detection of ROS in vivo.

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