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Abstract #3680

Hyperpolarized 13C-labeled 5-5-dimethyl-1-pyrroline-N-oxide for in vivo detection of reactive oxygen species

Keita Saito1, Shingo Matsumoto2, Deepak Sail3, Shun Kishimoto1, Hellmut Merkle4, Marcelino Bernardo5, Rolf Swenson3, James B. Mitchell1, and Murali C. Krishna1

1Radiation Biology Branch, National Cancer Institute, Bethesda, MD, United States, 2Graduate School of Information Science and Technology, Hokkaido University, Sapporo, Japan, 3Imaging Probe Development Center, National Heart, Lung, and Blood Institute, Rockville, MD, United States, 4National Institute of Neurological Disorder and Stroke, Bethesda, MD, United States, 5Molecular Imaging Program, National Cancer Institute, Bethesda, MD, United States

5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) is a spin trap agent used to detect reactive oxygen species (ROS). We synthesized 13C-labeled DMPO, and investigated feasibility of hyperpolarized 13C-DMPO to detect ROS generated in living animals. Hyperpolarized 13C-DMPO gave us a single peak at 76 ppm on the 13C-spectrum, and 13C-DMPO was distributed through the mouse body immediately after intravenous injection. The results indicate hyperpolarized 13C-DMPO provided sufficient magnitude of the 13C signal to be detected in the mouse body, and can be applied to some disease models to evaluate the capability for detection of ROS in vivo.

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