Abstract #3683

Multi-modal and multi-scale measurement of metabolism in breast cancer cells both in vitro and in vivo

Benjamin L Cox^1,2,3, Joseph M Szulczewski⁴, Kai D Ludwig¹, Erin B Adamson¹, David R Inman⁴, Stephen A Graves¹, Justin J Jeffery⁵, Jason D McNulty⁶, Patricia J Keely⁴, Kevin W Eliceiri^2,3,7, and Sean B Fain^1,7,8

¹Medical Physics, University of Wisconsin at Madison, Madison, WI, United States, ²Morgridge Institute for Research, Madison, WI, United States, ³Laboratory for Optical and Computational Instrumentation, University of Wisconsin at Madison, Madison, WI, United States, ⁴Cell and Regenerative Biology, University of Wisconsin at Madison, Madison, WI, United States, ⁵UW Carbone Cancer Center, Madison, WI, United States, ⁶Mechanical Engineering, University of Wisconsin at Madison, Madison, WI, United States, ⁷Biomedical Engineering, University of Wisconsin at Madison, Madison, WI, United States, ⁸Radiology, University of Wisconsin at Madison, Madison, WI, United States

A system and workflow for spatially registered in vivo optical microscopy, MRI, and PET of breast tumor metabolism is described. The system is coupled with a bioreactor designed to compare cellular metabolism in vitro using both optical microscopy and MR spectroscopy with the behavior of tumor cells in the in vivo tumor microenvironment. Results from enzyme reactions are shown, demonstrating the temperature control capabilities of the bioreactor. A proof of concept in vivo experiment is also described, with optical microscopy data of a mammary tumor acquired in conjunction with MRI and PET data, on the same animal.

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