Meeting Banner
Abstract #3963

In Vitro Oxygen-17 NMR Spectroscopy of Cellular Metabolism at Ultra High Field

Ruomin Hu1, Andreas Neubauer1, Jorge Chacón-Caldera1, Javier Uranga Solchaga1, Christian Schuch2, Tilo Gläser2, Cordula Nies3, Eric Gottwald3, Stefan Giselbrecht4, and Lothar R. Schad1

1Computer Assisted Clinical Medicine, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany, 2NUKEM Isotopes Imaging GmbH, Alzenau, Germany, 3Institute for Biological Interfaces 5, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany, 4Department of Complex Tissue Regeneration, MERLIN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, Netherlands

In this work we present a novel in vitro oxygen-17 NMR method using 17O-labeled glucose to investigate the metabolic process of cells cultivated in a MR-compatible microbioreactor. The metabolization of 17O-labeled glucose was demonstrated to produce MR-detectable H217O on the one hand and to not intervene with the inherent cellular physiology on the other hand, thus proving the method to serve as a neutral observation platform. The presented method has the potential to aid the modeling of fundamental physiological processes and to become a key element in cellular vitality assessment applications.

This abstract and the presentation materials are available to members only; a login is required.

Join Here