Abstract #4020

Visualizing lactate in the ischemic hindleg of mice using localized proton 2D correlation spectroscopy (UHf-P-COSY) at ultrahigh field.

Devashish Das¹, Andor Veltien¹, and Arend Heerschap¹

¹Radiology, Radboud University Nijmegen, Nijmegen, Netherlands

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Lactate is associated with mitochondrial and neuromuscular diseases. Therefore, its methylene (CH2) and methyl (CH3) proton assignment is necessary in the skeletal muscle. In particular for understanding mechanisms associated with the skeletal muscle ischemia in rodents. Unambiguous detection of lactate resonances in the ischemic and/or hypoxic rodent muscle remains challenging, however, necessary for the assessment of treatment in the dysfunctional muscle of transgenic animals. In vivo lactate resonances remain hidden under lipid pool. However, by tuning spinecho delays in the localized 2D proton correlation sequence we are able to separate lactate CH2 and CH3 protons from the lipid resonances in the F1 dimension of the 2D-spectrum.

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