The goal of this study was to measure the apparent diffusion coefficients (ADC) of five main metabolites in the human brain at 3 T using PRESS and STEAM sequences where measurement biases due to cross-terms were avoided by using positive and negative diffusion gradient polarity. This study shows that comparable trace/3 ADC values for total N-acetyl aspartate, total creatine, total choline, glutamate and myo-inositol can be successfully measured with both acquisition methods on a clinical scanner with STEAM providing slightly better quantification precision for J-coupled metabolites.
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