Meeting Banner
Abstract #0730

19F-perfluorocarbon-labeled human peripheral blood mononuclear cells can be detected in vivo using clinical MRI parameters in a therapeutic cell setting

Corby Fink1, Jeffrey Gaudet2, Matthew S Fox3, Shashank Bhatt4, Sowmya Viswanathan4, Michael Smith1, Joseph Chin5, Paula Foster2, and Gregory Dekaban1

1Microbiology and Immunology, Robarts Research Institute, London, ON, Canada, 2Medical Biophysics, Robarts Research Institute, London, ON, Canada, 3Medical Biophysics, Lawson Health Sciences Centre, London, ON, Canada, 4Cell Therapy Program, Krembil Research Institute, Toronto, ON, Canada, 5Urology, University of Western Ontario, London, ON, Canada

A major hurdle in advancing cell-based cancer vaccines is the inability to track where therapeutic cells migrate post-injection. We present the first study conducted in which primary Good Manufacturing Practice-grade 19Fluorine (19F) labeled all peripheral blood mononuclear cell lineages (PBMC) and 19F cellular MRI was used to track and quantify in vivo migration in a mouse model. Secondly, we present the highest sensitivity for 19F detection reported in the literature and the first time a 1.2cm deep injection of 19F-labeled PBMC using a small dual-tuned surface coil has been detected using a clinical scanner and MR protocol.

This abstract and the presentation materials are available to members only; a login is required.

Join Here