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Abstract #1674

Hemodynamic and neuronal response in rat under Isoflurane-supplemented Medetomidine sedation assessed by simultaneous BOLD fMRI and Ca2+ recordings

Timo van Alst1, Lydia Wachsmuth1, Franziska Albers1, Florian Schmid1, and Cornelius Faber1

1Department of Clinical Radiology, University of Muenster, Muenster, Germany

fMRI in rodents has become a widely used technique with various applications in preclinical research. In this context, most studies are performed with anesthetized animals. Medetomidine evolves as a frequently used sedative but long term stability is limited -prolonged stability can be achieved by addition of Isoflurane. Several recent fMRI studies have used this combination. We evaluated the effects of this combination of anesthetics using a multimodal setup of BOLD fMRI and Ca2+ recordings. Even small addition of Isoflurane resulted in a significant decrease of neural (Ca2+) and hemodynamic (BOLD) responses to a distinct electrical stimulus.

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