Chemical exchange effect can be evaluated by off-resonance spin-lock sequence (CESL), but it remains unclear whether the effect is accurately reflected. In this study, with the help of perturbation of longitudinal relaxation rate in rotating frame (PLRF) analysis, we quantitatively compared signal from CESL and chemical exchange saturation transfer (CEST) sequences and tested the condition for acquiring high-quality signal from CESL sequence which can accurately reflect chemical exchange effect.
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