The objectives are to investigate how the chemical exchange saturation transfer (CEST) signal measured at a given frequency is independent of the neighboring CEST signals. We measured the CEST signals from combinations of four metabolites at 3.5, 3, 2 and 1 ppm with 5 different powers and 3 different durations of presaturation pulse to investigate the parameter-dependence and interdependency of each CEST signal in phantoms. The CEST signal of glutamate was less impacted by concentration changes in other exchanging species by subtracting CEST signals at two different power levels.
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