Spinal cord injury (SCI) is a devastating damage that can result in permanent disabilities. MRI provides precise details of spinal cord post traumatic compression, which are useful for the diagnosis of the injury, surgical planning, post-surgical measurements and estimation long-term outcomes (Kramer, Freund, & Curt, 2014). In this study, 4 SCI mice were scanned at the 9.4T to acquire high-resolution images and diffusion tensor imaging at two b-values. The area of both white and gray matter were measured in several slices and compared to the pre-injury imaging. Spinal cord white and gray matters were also examined using DTI.