Abstract #2557

Histological Validation of Diffusion Basis Spectrum Imaging Using Autopsied Multiple Sclerosis Brain Specimens

Ajit George¹, Peng Sun², Anne H Cross³, Xiaojie Wang⁴, Carlos J Pérez-Torres⁵, and Sheng-Kwei Song⁶

¹Department of Radiology, Washington University, St. Louis, Saint Louis, MO, United States, ²Department of Radiology, Washington University, St. Louis, ³Department of Neurology, Washington University, St. Louis, MO, United States, ⁴Advanced Imaging Research Center, Oregon Health & Science University, OR, United States, ⁵Radiological Health Sciences, School of Health Sciences, Purdue University, West Lafayette, IN, United States, ⁶Department of Radiology, Washington University, St. Louis, MO, United States

In this study, we demonstrate diffusion basis spectrum imaging (DBSI) is able to detect, differentiate and quantify different coexisting pathologies, particularly axonal loss and demyelination within autopsied multiple sclerosis human brain specimens. We correlated the DBSI derived maps with quantitative histology maps generated by means of color based segmentation. DBSI-derived fiber fraction was seen to correlate with Bielschowsky’s silver stain for axonal integrity, and the DBSI-derived radial diffusivity negatively correlated with Luxol Fast Blue-Periodic Acid-Schiff (LFB-PAS) stain for myelin integrity.

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