We adopted a 2D heteronuclear single-quantum coherence (HSQC) MR spectroscopy method to detect in vivo 13C isotopomers in rat brain through exploiting the high sensitivity of 1H MRS. This method allows for in vivo detection of unique 13C-labeling patterns in brain metabolite pools during simultaneous infusion of different 13C-labeled substrates. We demonstrated that high-quality 2D HSQC MR spectra can be acquired in vivo in a time-resolved manner from rat brain during simultaneous infusion of [U-13C6]-glucose and [2-13C]-acetate. This method can be used to study with high accuracy neuronal and glial metabolism, and the contribution of alternate substrates to brain energy metabolism.
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