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Abstract #3092

Hyperpolarized 13C-MRI of DMPO and NAC for evaluating oxidative stress in living animal

Keita Saito1, Deepak Sail2, Burchelle N. Blackman2, Hellmut Merkle3, Rolf E. Swenson2, James B. Mitchell1, and Murali C. Krishna1

1National Cancer Institute, Bethesda, MD, United States, 2National Heart, Lung, and Blood Institute, 3National Institute of Neurological Disorder and Stroke

5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) is used to detect reactive oxygen species in vitro, and N-acetyl-L-cysteine (NAC) is an antioxidant. We synthesized 13C-labeled DMPO and NAC, and investigated feasibility of hyperpolarized 13C-DMPO and 13C-NAC for evaluating oxidative stress in mice. Hyperpolarized 13C-DMPO and 13C-NAC provided a single peak at 76 ppm and 174 ppm, respectively, and the T1 relaxation time was sufficiently long to apply them for mouse imaging. The signals 13C-DMPO and 13C-NAC were also detected in a mouse body after intravenous injection. The results showed 13C-DMPO and 13C-NAC can be applied to some disease models to evaluate oxidative stress in vivo.

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