Diffusion-weighted (DW) MRI of the prostate has increased the diagnostic accuracy for the detection of prostate cancer. However, acquisition time of DWI is still relatively long. Therefore, we evaluated the feasibility of simultaneous multi-slice (SMS) DWI for accelerated MRI of the prostate. Qualitative and quantitative image analyses in phantom, volunteer and patient measurements revealed similar image quality for DWISMS as compared to standard DWI sequences. Thus, DWISMS seems feasible for clinical routine in order to optimize patient throughput and economic efficiency, which is desirable, due to the recent implementation of prostate MRI into clinical guidelines and the expected increase in patient numbers.