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Abstract #3438

Adipose tissue and ectopic fat responses to an extended fast in healthy male adults

Alison Sleigh1,2, Ajay Thankamony3, Albert Koulman4, Vlada Bokii1, Graham J Kemp5, Leanne Hodson6, and David B Dunger3

1Wolfson Brain Imaging Centre, University of Cambridge, Cambridge, United Kingdom, 2NIHR/WT Clinical Research Facility, Cambridge University Hospitals NHS Foundation Trust, Cambridge, United Kingdom, 3Department of Paediatrics, University of Cambridge, Cambridge, United Kingdom, 4MRC Human Nutrition Research, Cambridge, United Kingdom, 5Department of Musculoskeletal Biology, University of Liverpool, Liverpool, United Kingdom, 6Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, Oxford, United Kingdom

We have investigated the response of different fat deposits to a 28 h fast in healthy nonobese males and shown significant increases in CH2 IMCL in both the soleus and tibialis anterior muscles, and significant decreases in subcutaneous fat. Increases in TA and SOL IMCL correlated with oleic and palmitic acid FFA concentrations respectively. The compositional change of IMCL during the fast related inversely to the baseline composition, suggesting a selective efflux of unsaturated shorter chain IMCLs for preferential oxidation, which is in agreement with rat biopsy data, and supportive of the idea of IMCL as a flexible lipid store.

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