Non-alcoholic steatohepatitis (NASH) is associated with metabolic syndrome as a result of insulin resistance and the accumulation of lipid droplets within hepatocytes. There is a need to diagnose and to accurately assess the progressive severity of hepatic steatosis with non-invasive biomarkers that are distinguishable from those in non-alcoholic fatty liver disease (NAFLD). Our findings demonstrate that fatty acid metabolism (saturated- and unsaturated-fatty acids) of hepatic steatosis induced by a methionine-choline diet can be distinguished from progressive NAFLD by using high-resolution magnetic resonance spectroscopy (MRS).
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