The β‑alanine loaded hollow mesoporous silica nanospheres (AMSNs) with a characteristic MRS spectrum are successfully synthesized and used for contrast enhanced magnetic resonance spectroscopy (MRS) imaging of brain glioma. Material characterizations, in vitro and in vivo cytotoxicity studies and contrast enhanced MRS imaging of both subcutaneously transplanted glioma and in situ glioma are conducted with the synthesized AMSNs.
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