We propose that a two-compartment model of the evolution of signal phase, processed using Frequency Difference Mapping, could be applied to study venous oxygenation and architecture at the microscopic scale. This study demonstrates proof-of-concept, quantifying Yv in large vessels, and tests the sensitivity of these values to hyperoxia. We discuss how this new method can be adapted to probe venous microstructure within grey matter, providing more sensitive measures of brain tissue function.
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