In the current study we tested if accumulation of non-radioactive 2-deoxy-D-glucose (2DG)-conjugated magnetonanoparticles (2DG-MNP) can be used as a measure of metabolic activity in glioblastoma. To quantify 2DG-MNP uptake across brain ΔR2 maps are proposed. The difference in R2 relaxation rates prior to and at different times following 2DG- or unlabeled (plain) MNP administration represents ΔR2. Significant changes in ΔR2 between brain and glioblastoma, reflecting increased metabolic rate of glioblastoma were found for glucose labeled MNP and not for plain-MNP. Our results suggest that 2DG-MNP have potential to be utilized in metabolic imaging as non-radioactive 2-18[F]-fluoro-deoxy-D-glucose analogue.