The purpose of this study is to assess the CEST effect and contrast of various brain regions in normal for phase cycle-CEST MR imaging. Subjects were five healthy volunteers. All slice positions were set on the nucleus basalis level. ROIs were set as thalamus, frontal, occipital, putamen, and gray matter on right and left brain hemispheres of each subject There was no significant difference in mean MTRasym values among each brain region (P > 0.01 for all). CEST effects for normal brain tissue had no dependency on region and/or left-right hemispheres.