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Abstract #4652

A metabolic study of hypoxic ischemia during mouse brain development using hyperpolarized 13C

Yiran Chen1,2, Byongsop Lee3,4, Robert Bok1, Ilwoo Park1, Subramaniam Sukumar1, R Ann Sheldon3,4, A James Barkovich1,4, Donna M Ferriero3,4, and Duan Xu1,2

1Department of Radiology and Biomedical Imaging, UCSF, San Francisco, CA, United States, 2Joint UCSF/UC Berkeley Graduate Group in Bioengineering, UCSF, San Francisco, CA, United States, 3Department of Neurology, UCSF, San Francisco, CA, United States, 4Department of Pediatrics, UCSF, San Francisco, CA, United States

In this study, we applied dynamic nuclear polarization (DNP) technique to investigate C1 labeled 13C pyruvate to lactate conversion on hypoxic ischemia (HI) injured neonatal mouse brains during development. Our results showed that lower pyruvate level and higher lactate to pyruvate ratio on the injured hemisphere in comparison to the non-injured hemisphere at the day of injury (P10). This difference narrows as the brain matures. With this technique, we are able to examine individuals’ response to HI in vivo during brain development.

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