Diffusion fMRI (dfMRI) is highly promising for improving the detection of active regions with higher spatial accuracy, as well as for its potential of resolving faster dynamics than its BOLD counterpart. To test this hypothesis, we compared BOLD and dfMRI in the auditory pathway of the mouse, which exhibits clear tonotopy in electrophysiology. Our findings suggest that dfMRI activation maps are more localized and are in agreement with the expected area of activation in the inferior colliculus; dfMRI signals were also ~3s faster than BOLD signals. These results are expected to enable brainwide characterization of auditory reorganization, function, and plasticity.
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