The application of phase cycling scheme in combination with interleaved off-odd switch has been suggested for MEGA-PRESS method in many previous reports but the effect of phase cycling schemes on the spectral shape of GABA peak and on the GABA quantification has not been studied yet. Here, performance of phase cycling by different number of steps were and by modified interleaved scheme were compared. The results show that phase cycling is necessary to be applied prior interleaved on-off switch and minimum shape cycling steps are suggested to minimize subtraction artifacts possibly resulting from on-off frequency drift and motion.
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