Quantitative magnetization transfer (qMT) and multi-exponential T2-based myelin water imaging (MWI) are commonly-used MRI methods to quantify myelin content. Ex vivo MRI remains an essential step for validating these quantitative in vivo MRI biomarkers. However, ex vivo tissue is often preserved using formalin, which cross-links proteins and directly impacts these methods. We performed qMT and MWI on human spinal cord tissue before and after formalin fixation to quantify the effect of fixation on these biomarkers. QMT bound pool fraction (F) increased by 37.5% and MWI myelin water fraction (MWF) increased by 35.5-38.6%, but myelin-related image contrast was preserved.
How to access this content:
For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.
After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.
After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.
Keywords