Alzheimer’s disease (AD) is a degenerative disorder and the most common cause of dementia. The hallmark of AD is the accumulation of Aβ-plaque in the subject brain. At present there is no treatment for AD. Extracts of Withania somnifera (WS) roots has been shown to promote neurite outgrowth and improve learning and memory in AD mice. The current study examine the effects of WS on neurometabolism in AβPP-PS1 mouse model of AD by using 1H-[13C]-NMR spectroscopy in conjunction with infusion of [1,6-13C2]glucose. Our findings indicate that WS improved learning and memory, and ameliorate the neurometabolism in AβPPPS1 mice.