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Abstract #0790

3D microscopy with CLARITY on human brain tissue: a tool for informing and validating MRI-based histology

Markus Morawski1, Evgeniya Kirilina2,3, Nico Scherf2, Carsten J├Ąger2, Katja Reimann1, Robert Trampel2, Filippos Gavriilidis2, Stefan Geyer2, Bernd Biedermann1, Thomas Arendt1, and Nikolaus Weiskopf2

1Paul Flechsig Institute for Brain Research, University of Leipzig, Leipzig, Germany, 2Department of Neurophysics, Max Planck Institute for Human Cognitive and Brain Sciences, Leipzig, Germany, 3Center for Cognitive Neuroscience Berlin, Free University Berlin, Berlin, Germany

Recent developments of methods for mapping tissue microstructure with MRI require histological 3D data for validation. Here, CLARITY on post mortem human brain was adapted for this purpose. We demonstrated clearing of up to 8 mm thick samples, 3D microscopy of up to 5 mm thick samples, and the application of multiple stains including markers for neurons, glia, and fibers. The result is a detailed histology-based characterization of cyto- and myelo-architectonics within a volume corresponding to a typical MRI voxel. This approach promises to help integrate MRI-based histology and optical microscopy in 3D, and enable the further development and validation of in vivo histology using MRI.

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