Magnetic Resonance Fingerprinting (MRF) allows the quantification of multiple tissue parameters with high efficiency. Previously, we developed an MRF based 31P spectroscopic method for fast and robust measurement of ATP synthesis via creatine kinase (CK). In the current study, we explored the potential of combining the CK-MRF method with fast imaging for metabolic mapping of the CK reaction rate in small laboratory animals. CK-MRF imaging was performed in the hindlimb of four rats. CK rates of different muscle compartments were compared.