Abstract #0952

Multiscale measurement of the effects of glucose starvation on 4T1 murine breast cancer cells using an MRI and optical microscopy compatible bioreactor

Benjamin L Cox^1,2,3, Sarah Erickson-Bhatt^2,3,4, Joseph M Szulczewski^3,4, Kai D Ludwig¹, Erin B Adamson¹, Robert A Swader², Suzanne M Ponik⁴, Kevin W Eliceiri^1,2,3,5, and Sean B Fain^1,6

¹Medical Physics, University of Wisconsin - Madison, Madison, WI, United States, ²Morgridge Institute for Research, Madison, WI, United States, ³Laboratory for Optical and Computational Instrumentation (LOCI), University of Wisconsin - Madison, Madison, WI, United States, ⁴Cell and Regenerative Biology, University of Wisconsin - Madison, Madison, WI, United States, ⁵Biomedical Engineering, University of Wisconsin - Madison, Madison, WI, United States, ⁶Radiology, University of Wisconsin - Madison, Madison, WI, United States

The design and application of a novel bioreactor capable of facilitating both magnetic resonance spectroscopy (MRS) and optical fluorescence microscopy for complementary metabolic information is described. Fluorescence lifetime imaging (FLIM) of nicotinamide adenine dinucleotide (NADH) and hyperpolarized [1-¹³C] pyruvic acid (PA) MRS were performed on 3D cell cultures of 4T1 murine breast cancer cells to study the effects of glucose starvation across cellular and population scales. The system provides a novel test-bed for simulating cell-matrix and cell-cell interactions in a 3D microenvironment for investigating multi-scale cellular metabolism in vitro.

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