Abstract #0952

Multiscale measurement of the effects of glucose starvation on 4T1 murine breast cancer cells using an MRI and optical microscopy compatible bioreactor

Benjamin L Cox^1,2,3, Sarah Erickson-Bhatt^2,3,4, Joseph M Szulczewski^3,4, Kai D Ludwig¹, Erin B Adamson¹, Robert A Swader², Suzanne M Ponik⁴, Kevin W Eliceiri^1,2,3,5, and Sean B Fain^1,6

¹Medical Physics, University of Wisconsin - Madison, Madison, WI, United States, ²Morgridge Institute for Research, Madison, WI, United States, ³Laboratory for Optical and Computational Instrumentation (LOCI), University of Wisconsin - Madison, Madison, WI, United States, ⁴Cell and Regenerative Biology, University of Wisconsin - Madison, Madison, WI, United States, ⁵Biomedical Engineering, University of Wisconsin - Madison, Madison, WI, United States, ⁶Radiology, University of Wisconsin - Madison, Madison, WI, United States

The design and application of a novel bioreactor capable of facilitating both magnetic resonance spectroscopy (MRS) and optical fluorescence microscopy for complementary metabolic information is described. Fluorescence lifetime imaging (FLIM) of nicotinamide adenine dinucleotide (NADH) and hyperpolarized [1-¹³C] pyruvic acid (PA) MRS were performed on 3D cell cultures of 4T1 murine breast cancer cells to study the effects of glucose starvation across cellular and population scales. The system provides a novel test-bed for simulating cell-matrix and cell-cell interactions in a 3D microenvironment for investigating multi-scale cellular metabolism in vitro.

How to access this content:

For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.

After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.

After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.

Click here for more information on becoming a member.