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Abstract #1114

Simultaneous fMRI and multispectral fiber-photometry for concurrent triple-modality measurement of genetically encoded calcium activity, CBV and BOLD

Wei-Ting Zhang1, Tzu-Hao Chao1, Sung-Ho Lee1, Brittany Michelle Katz1, Esteban Oyarzabal1, Guohong Cui2, and Yen-Yu Ian Shih1

1Biomedical Research Imaging Center and Department of Neurology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States, 2Neurobiology Laboratory, National Institute of Environmental Health Sciences, NIH, RTP, NC, United States

We demonstrate the experimental setup and the use of multispectral fiber-photometry during fMRI in vivo for concurrent measurements of GCaMP6f, CBV, and BOLD. The fluorescent intensity of GCaMP6f and Rhodamine B, a red fluorescent dye which represents CBV, increased during spontaneous motion in the freely moving animal. GCaMP6f, CBV, and BOLD increased when forepaw electric stimulation was given in the simultaneous fMRI and fiber-photometry. We conclude that simultaneous multispectral fiber-photometry and functional MRI is a powerful tool which has an advantage in both temporal and spatial dimensions. Rhodamine B could be a surrogate CBV indicator in the regions of interest.

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