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Abstract #1263

Principles for measuring whole kidney nephron endowment in mice with in vivo MRI

Edwin J. Baldelomar1, Jennifer R. Charlton2, and Kevin M. Bennett3

1Physics, University of Hawaii at Manoa, HONOLULU, HI, United States, 2Pediatrics, University of Virginia, Charlottesville, VA, United States, 3Biology, University of Hawaii at Manoa, Honolulu, HI, United States

Nephron endowment is a strong predictor for renal health and nephron loss is a hallmark of early development of chronic kidney disease, but there are no techniques to measure nephron endowment in vivo. Here, we performed CFE-MRI in rats and mice to determine the limits of in vivo measurements of nephron endowment in vivo. We correlated in vivo measurements with measurements from ex vivo, high-resolution images of same kidneys. This work lays the foundation for consistent and accurate whole kidney glomerular number and size measurements in the mouse and rat kidney, in vivo.

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