Glaucoma is a worldwide leading cause of irreversible vision loss characterized by degeneration of retinal ganglion cells. The damage can be found in visual pathway beyond retina and optic disc to visual cortex. Diffusion tensor MR imaging (DTI) is widely used for evaluation of early microstructural change in the brain parenchyma. Here we reported abnormal change of the optic radiation in advanced bilateral glaucoma patients using DTI as compared to the age-matched normal control subjects. The obtained DTI parameters may serve as potential quantitative imaging biomarkers to provide complementary indication of the disease condition in glaucoma.