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Abstract #1999

Influence of Different Anesthesia Protocols on Cerebrovascular Reactivity and Cerebral Blood Flow measured by Pseudo-continuous Arterial Spin Labeling

Marc Derieppe1, Leon Munting1, Ernst Suidgeest1, and Louise van der Weerd1

1Department of Radiology, Leiden University Medical Center, Leiden, Netherlands

Anesthesia protocols in animal studies greatly influence cerebral hemodynamics, so it is critical to devise standardized protocols in order to provide reproducible and comparable Cerebral Blood Flow (CBF) and Cerebral Vascular Reactivity (CVR) in different mouse strains or models. We compared strain-dependent sensitivity towards different anesthesia protocols for vascular reactivity experiments (high-dose isoflurane, medetomidine, low-induction dose isoflurane and high-dose isoflurane in intubated and mechanically ventilated mice), using pseudo-Continuous ASL (pCASL) and discuss the relative performance of these protocols.

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